Enzyme Tool Box Sucrose Synthase + Nucleotide sugar pathway enzymes

Enzyme Tool Box L. & F. Biomaterialien

Multi-Enzyme Cascade Synthesis of Nucleotide Sugars

Multi-Enzyme Cascade Synthesis of Nucleotide Sugars L. & F. Biomaterialien

We have developed modular enzyme systems for the synthesis and in situ regeneration of different nucleotide sugars including enzymes from different biosynthetic pathways in plants and microorganisms [ 1, 3, 10, 18, 22, 23, 26, 33, 35, 37, 44, 45, 46, 49, 77, 84 ].

Enzyme module dTDP-Rhamnose

Synthesis of dTDP-Rhamnose and In-situ Regeneration of UDP-Glucuronic Acid L. & F. Biomaterialien

With the plant enzyme Sucrose Synthase (SuSy) we have established a novel access to nucleotide (deoxy)sugars starting from sucrose and nucleoside monophosphates[4, 5, 7, 12, 13, 16, 19, 23, 24, 26, 27, 28, 29, 30, 45, 46, 66, 84]. An illustrative example is the g-scale synthesis of dTDP-6-deoxy-4-ketoglucose, which is a central intermediate in the complex biosynthetic pathways of D- and L-deoxysugars [46]. The combination of further enzymes succeeded in the synthesis of complex dTDP-deoxyhexoses from sucrose, e.g. dTDP-L-Rhamnose [46] . In summary, we have established and optimized enzyme module systems for 16 nucleotide sugars. The enzyme modules are further utilized for the in vitro characterization of recombinant bacterial glycosyltransferases for their potential to engineer the glycosylation of macrolide structures for the development of novel antibiotics [45, 49, 50].