Methods

  • Gene expression in microbial hosts
  • Enzyme production by fermentation processes
  • Enzyme purification (chromatography)
  • Biochemical enzyme characterization (kinetics, stability, substrate spectrum)
  • Proteinchemical characterization of enzymes (SDS-PAGE, Western-Blot)
  • High-throughput analysis and optimization of multi-enzyme biocatalytic processes
  • Semi-preparative synthesis and purification of glycoconjugates
  • Isolation and characterization of glycoconjugates (HPLC, CE, HPLC/ESI-MS, NMR and high resolution MS in cooperation)